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The NIH AIDS Reagent Program has been transitioned to the NIH HIV Reagent Program which is managed by American Type Culture Collection (ATCC). We want to ensure your continued use of the materials within the NIH HIV Reagent Program with minimal impact to you. The NIH HIV Reagent Program materials are provided at no cost to registered researchers (shipping charges apply).
J-Lat cells were generated by infecting Jurkat cells with an HIV-1 pseudotyped virus, where Nef was replaced with GFP, and clones were characterized (ARP-9846 to ARP-9850). Similar latent cells were also established with a simpler retroviral vector, Tat-IRES-EGFP, under the control of LTR, and clones were characterized (ARP-9851 to ARP-9856). All clones can be activated by most Latency Reversing Agents. For analysis, GFP can be measured by flow cytometry or p24 ELISA of culture supernatants.
Red-Green-HIV-1 (RGH) molecular clone (ARP-12427), is HIV-1 B-LAI Δenv double-labeled with eGFP, as an in-frame Gag fusion, and CMV-driven mCherry, located in place of Nef. Therefore, it allows the detection of infected cells independently of long terminal repeat (LTR) activity and serves as a sensitive tool to help in analyzing HIV-1 latency on a single-cell level. Related materials include ARP-12428 (pRGH-Integrase defective), ARP-12429 (pRGH-ΔCMV) and ARP-12430 (pRGH-ΔU3(3’).
This panel of full-length functional gp160 expression vector clones from 56 international HIV-1 primary isolates representing six clades (A, B, C, D, AE, and AG) is a great tool for vaccine and functional immune response studies, which can be used to generate pseudoviruses and naturalization assays. These clones are available in the NIH HIV Reagent Program catalog individually (ARP-13489 to ARP-13523) or as part of a panel set (ARP-13467).
Peptide pool, HIV-1 subtype B Env (ARP-12540), Gag (ARP-12425), Nef (ARP-12545), Pol (ARP-12438), Vpr (ARP-12860) and Rev (ARP-6445) are of the most requested reagents in the HRP catalog. These peptides are produced as 15-mers with 11 aa overlaps so that T-cell stimulation is optimized while the chance of missing T-cell epitopes is minimized. Peptide arrays for the same HIV-1 viral proteins are also available (ARP-9480, ARP-8117, ARP-5189, ARP-6208, ARP-6447).
Additional SIV and SHIV stocks produced for NIAID are available through Quality Biological. Click here for a list of items.
For information and forms regarding registration with the NIH HIV Reagent Program, click here.
After your registration application has been approved by NIAID and ATCC, you will receive a login name and password. After logging in, find the reagent you need using the search function. Select the items from the search results to see the product detail pages. After you read the product detail page description, click the "Add to cart" button to place the item into your shopping cart. The NIH HIV Reagent Program materials are provided at no cost to registered researchers (shipping charges apply). For additional information, click here.
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The NIH HIV Reagent Program acquires, authenticates, produces and distributes a variety of materials within several biosafety levels and classifications. We understand that some researchers may only choose to order non-infectious research grade reagents through the NIH HIV Reagent Program.
The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the NIH HIV Reagent Program and reagents through questions and answers.
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