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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-11401 was derived from the pConBgp160-opt clone (ARP-11402) by truncation of the subtype B env gene. The env gene was truncated by introducing a stop codon immediately after the transmembrane domain (SIVNR). A Kozak sequence (GCCGCCGCC) was introduced upstream of the ATG start codon. The final construct was ligated into pcDNA3.1(-) (Invitrogen™) expression vector. The combined size of vector and insert is approximately 7500 base pairs including the approximately 2100 base pair insertion and contains genes for ampicillin and neomycin resistance. The size of the translated Env protein is approximately 700 amino acids.
pConBgp160-opt clone and its derivatives, pConBgp120-opt (ARP-11400), pConBgp145-opt, pConBgp160-201NS-opt (ARP-11403) and pConBgp160-UNC-opt (ARP-11404), can be used for studies of protein expression and function and generation of subunit vaccines against subtype B viruses.
Note: The recommended host strain is JM109. For PCR amplification, it is recommended to use the GC-RICH PCR System (Roche) or another kit specialized for performing difficult amplifications.
Each vial of ARP-11401 contains approximately 5 µg of dried, purified DNA stabilized in DNAstable®Plus (Biomatrica®). Please refer to the appropriate data sheet for lot-specific information.
Kothe, D. L., et al. “Antigenicity and immunogenicity of HIV-1 consensus subtype B envelope glycoproteins.” Virology 360 (2007): 218-234. PubMed: 17097711.
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