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limit per order for this item is 1. This
item can be ordered twice a year. Orders over this limit will be sent to NIAID
for approval before shipment.
ARP-11507 is plasmid that
expresses HIV-1 subtype C Env gp160, Rev and Vpu derived from the genomic DNA of infected peripheral
blood mononuclear cells (PBMCs) from a newly acquired, heterosexually
transmitted HIV-1 infection in Pune, India (GenBank: EF117273). The construct consists of approximately 9000
base pair pcDNA 3.1D/V5-His TOPO® (Invitrogen™) backbone
with an approximately 3200 base pair insertion and contains the genes for ampicillin
and neomycin resistance.
Co-transfection with this env defective
viral molecular clone will yield pseudovirus that utilizes CCR5 as a co-receptor.
ARP-11507 is part of a standard panel of Subtype C Indian Env clones (ARP-11672)
Alternate names for this plasmid are HIV-25925-2, clone 22 and HIV-25925-2.22.
Note: Plasmids can be propagated in STBL2 cells and
grown at 37°C. Larger plasmids may benefit from growth at 30°C.
Each vial of ARP-11507 contains approximately 20 µg of dried, purified DNA stabilized in DNAstable®Plus (Biomatrica®). Please refer to the appropriate data sheet for
Kulkarni, S. S., et al. “Highly Complex Neutralization
Determinants on a Monophyletic Lineage of Newly Transmitted Subtype C HIV-1 Env
Clones from India.” Virology 385 (2009): 505-520. PubMed:19167740.
Scientists at for-profit
institutions or who intend commercial use of this reagent must contact the Duke
University-Office of Corporate Research Collaborations, Email: email@example.com,
before the reagent can be released. Please specify the name and a description
of the intended use of the reagent.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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