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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-103 is a human embryonic kidney cell line (HEK-293) isolated from primary human embryonic kidney tissue and transformed by
sheared human adenovirus type 5 DNA.
ARP-103 contains the
E1A/E1B genes of human adenovirus type 5 integrated into chromosome 19. The
cell origin is unclear. While originally thought to be epithelial cells, data
suggest they are of neuronal or adrenal gland origin.
Growth Characteristics: ARP-103 grows in a monolayer and double every 20 hours. The culture should be split in a ratio of 1:10. Cells should be passaged when almost confluent at 1:6.
The recommended propagation medium is DMEM supplemented with 10% fetal bovine serum.
Tests for bacteria, fungi and mycoplasma were negative.
Each vial of ARP-103 contains approximately 9.9 × 106 cells in 0.8 mL of freeze medium. Post-thaw viability was 93%.
Graham, F. L., et al. “Characteristics of a Human
Cell Line Transformed by DNA from Human Adenovirus Type 5.” J. Gen. Virol. 36 (1977), 59-74.PubMed: 886304.
Lin, Y. C., et al. “Genome Dynamics of the Human Embryonic Kidney
293 Lineage in Response to Cell Biology Manipulations.” Nat. Commun. 5
(2014): 4767. PubMed: 25182477.
Shaw, G., et al. “Preferential Transformation of Human Neuronal
Cells by Human Adenoviruses and the Origin of HEK 293 Cells.” FASEB J.
16 (2002): 869-871. PubMed: 11967234.
Stepanenko, A. A. and V. V. Dmitrenko. “HEK293 in Cell Biology
and Cancer Research: Phenotype, Karyotype, Tumorigenicity, and Stress-Induced Genome-Phenotype
Evolution.” Gene 569 (2015): 182-190. PubMed: 26026906.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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