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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. ARP-103 is a human embryonic kidney cell line (HEK-293) isolated from primary human embryonic kidney tissue and transformed by sheared human adenovirus type 5 DNA.
ARP-103 contains the E1A/E1B genes of human adenovirus type 5 integrated into chromosome 19. The cell origin is unclear. While originally thought to be epithelial cells, data suggest they are of neuronal or adrenal gland origin.
Growth Characteristics: ARP-103 grows in a monolayer and double every 20 hours. The culture should be split in a ratio of 1:10. Cells should be passaged when almost confluent at 1:6.
The recommended propagation medium is DMEM supplemented with 10% fetal bovine serum. Tests for bacteria, fungi and mycoplasma were negative.
Each vial of ARP-103 contains approximately 9.9 × 106 cells in 0.8 mL of freeze medium. Post-thaw viability was 93%.
Graham, F. L., et al. “Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5.” J. Gen. Virol. 36 (1977), 59-74.PubMed: 886304. Lin, Y. C., et al. “Genome Dynamics of the Human Embryonic Kidney 293 Lineage in Response to Cell Biology Manipulations.” Nat. Commun. 5 (2014): 4767. PubMed: 25182477. Shaw, G., et al. “Preferential Transformation of Human Neuronal Cells by Human Adenoviruses and the Origin of HEK 293 Cells.” FASEB J. 16 (2002): 869-871. PubMed: 11967234. Stepanenko, A. A. and V. V. Dmitrenko. “HEK293 in Cell Biology and Cancer Research: Phenotype, Karyotype, Tumorigenicity, and Stress-Induced Genome-Phenotype Evolution.” Gene 569 (2015): 182-190. PubMed: 26026906.
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