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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-11857 is a Jurkat T cell-based reporter
cell line that expresses CD4 and CXCR4, and is derived as a spontaneously
occurring sub-clone of JLTRG cells. In the absence of human
immunodeficiency virus 1 (HIV-1) infection or HIV-1 Tat expression, the cells
exhibit no green fluorescence protein (eGFP) expression. JLTRG cells cannot be
infected with R5 tropic virus.
ARP-11857 expresses eGFP as a direct and quantitative marker
of HIV-1 infection or HIV-1 Tat expression. Expression of both CD4 (95%) and
CCR5 (77%) has been confirmed by fluorescence-activated cell sorting (FACS).
Growth characteristics: ARP-11587 has a doubling time
of 24 to 48 hours.
Growth Media: RPMI 1640 supplemented with 10% fetal
ARP-11587 is negative for bacteria, Mycoplasma and fungi.
Each vial of ARP-11587 contains approximately 4 × 106 cells in RPMI 1640 supplemented with 40% fetal bovine
serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data
sheet for lot-specific information.
Kutsch, O., et al. “Bis-Anthracycline Antibiotics
Inhibit Human Immunodeficiency Virus Type 1 Transcription.” Antimicrob.
Agents Chemother. 48 (2004): 1652-1663. PubMed:
Ochsenbauer-Jambor, C., et al. “T-Cell Line for
HIV Drug Screening Using EGFP as a Quantitative Marker of HIV-1 Replication.” Biotechniques
40 (2006): 91-100.PubMed: 16454046.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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