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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. This human T lymphoblastoid cell line (originally called CEM-T4) is a naturally isolated subclone of the CEM line with high levels of surface CD4 expression.
Growth Characteristics: Cells are grown in suspension with a lymphoblast-like morphology. An inoculum of 105 cells per mL will increase four to five fold in 4 to 5 days when incubated at 37°C, providing pH is maintained at 7.0 and fresh medium is added every other day. Maintenance of the cell population at 106 cells per mL is optimal for growth.
Propagation Medium: Minimal Essential Medium (MEM) supplemented with 10% fetal bovine serum and without antibiotics ARP-117 is negative for mycoplasma, bacteria and fungi. Each vial of ARP-117 contains approximately 3.8 x 106 cells in Minimal Essential Medium (MEM) supplemented with 10% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for more information.
Foley, G. E., et al. “Continuous Culture of Human Lymphoblasts from Peripheral Blood of a Child with Acute Leukemia.” Cancer 18 (1965): 522-529. PubMed: 14278051.
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