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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-1296 is a culture of BF24 cells, a THP-1 derivative that contains stably integrated, silent copies of the human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR) promoter linked to the CAT gene.
The BF24 cell line was generated by infection of THP-1 cells with a helper-free recombinant retroviral vector containing the HIV-1 LTR-CAT gene construct. BF24 was selected in G418 under limiting dilution and is a sensitive indicator cell line for HIV-1 Tat. When infected by HIV-1, BF24 produces high levels of chloramphenicol acetyl transferase (CAT). Contains LTR sequences to +80 in the R region. ARP-12296 contains the entire U3 region, but lacks U5 sequences.
Growth Characteristics: Split cells twice weekly at a ratio of 1:3. BF24 cells are stable and do not need to be maintained in selection medium. If growth in selection medium is desired, propagation medium containing 700 µg per mL G418 should be used. Wash the thawed cells in propagation medium and centrifuge for 10 minutes at 1000 rpm before seeding the cells in a culture flask.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum
ARP-1296 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-1296 contains
approximately 6 × 106 cells in RPMI 1640 supplemented with 20% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the
appropriate data sheet for lot-specific information.
B. K. and G. Pavlakis. “A Quantitative Bioassay for HIV-1 Based on Trans Activation. Science 239 (1988):
Schwartz, S., et
al. “Rapidly and Slowly Replicating Human Immunodeficiency Virus Type 1 Isolates
can be Distinguished According to Target-Cell Tropism in T-Cell and Monocyte Cell
Lines.” Proc. Natl. Acad. Sci.USA 86 (1989): 7200-7203. PubMed: 2789383.
An NCI patent application has been filed on the use of the cell line H938. Corporate requests should be directed in writing to: B.K. Felber or G.N. Pavlakis, National Cancer Institute, FCRDC, ABL-Basic Research Program, P.O. Box B/Building 539, Room 121, Frederick, Maryland 21702-1201. Phone: (301) 846-1474, FAX (301) 846-5991.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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