Disclaimer: You are now leaving www.HIVReagentProgram.org and are going to a website that is not operated by NIH HIV Reagent Program. We are not responsible for the content or availability of linked sites.
ABOUT THIRD PARTY LINKS ON OUR SITE: NIH HIV Reagent Program offers links to other third party websites that may be of interest to our website visitors. The links provided in our website are provided solely for your convenience and may assist you in locating other useful information on the Internet. When you click on these links, you will leave the NIH HIV Reagent Program website and will be redirected to another site. These sites are not under the control of NIH HIV Reagent Program. NIH HIV Reagent Program is not responsible for the content of linked third party websites. We are neither an agent for these third parties nor do we endorse or guarantee their products. We make no representation or warranty regarding the accuracy of the information contained in the linked sites. We suggest that you always verify the information obtained from linked websites before acting upon this information. Please read third party privacy and security policies closely as these may be different than NIH HIV Reagent Program policies. If you have any questions or concerns about the products and services offered on linked third party websites, please contact the third party directly.
-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. ARP-1301 is the HeLa CD4+ cell line infected with the human immunodeficiency virus 1 (HIV-1) lymphadenopathy-associated virus (LAV) established by infecting HeLa CD4+ cells (ARP-154) with HIV-1 LAV followed by subcloning.
ARP-1301 expresses high levels of all viral proteins, and > 90% of them express cell surface gp120. Syncytia formation does not occur. The cells are suitable targets for assays of cell killing because they are susceptible to killing by allogeneic CTL, are easily labeled with 51Cr, and exhibit a low spontaneous release of 51Cr. The cells release infectious viral particles.
ARP-1301 is also referred to as HeLa/LAV. Growth Characteristics: Cells are mostly adherent, with some clumping. Variable morphology. Cultures should be maintained at a density of 105 to 106 cells per mL. Growth Medium: DMEM supplemented with 10% fetal bovine serum
ARP-1301 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-1301 contains approximately 2.6 × 106 cells in fetal bovine serum containing 10% dimethyl sulfoxide (DMSO).
Please refer to the attached file(s) for more information.
If shipping to the U.S. State of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers. While we use reasonable efforts to include accurate and up-to-date information on this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.