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-100°C or colder, preferably in the vapor phase
of a liquid nitrogen freezer
Quantity limit per order
for this item is 1. This item can be ordered twice a year. Orders over this
limit will be sent to NIAID for approval before shipment.
ARP-1301 is the HeLa CD4+ cell line infected
with the human immunodeficiency virus 1 (HIV-1) lymphadenopathy-associated
virus (LAV) established by infecting HeLa CD4+ cells (ARP-154) with HIV-1 LAV
followed by subcloning.
ARP-1301 expresses high levels of all viral proteins, and > 90% of them
express cell surface gp120. Syncytia formation does not occur. The cells are
suitable targets for assays of cell killing because they are susceptible to
killing by allogeneic CTL, are easily labeled with 51Cr, and exhibit a low spontaneous release of 51Cr. The cells release infectious viral particles.
ARP-1301 is also referred to as HeLa/LAV.
Growth Characteristics: Cells are mostly adherent, with some clumping.
Variable morphology. Cultures should be maintained at a density of 105 to 106 cells per mL.
Growth Medium: DMEM supplemented with 10% fetal bovine serum
ARP-1301 is negative
for bacteria, fungi and Mycoplasma.
Each vial of ARP-1301 contains approximately 2.6 × 106 cells in fetal bovine serum containing 10% dimethyl
Please refer to the attached file(s) for more information.
If shipping to the U.S. State of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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