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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-3553 is a 293 subcloned cell line that supports Kaposi's sarcoma-associated herpesvirus (KSHV) replication.
ARP-3553 is a subgroup of the human embryonal-kidney epithelial cell line 293 transformed by sheared human adenovirus DNA. These cells support KSHV replication in vitro when cocultured with Kaposi’s sarcoma cell lines. It has been used in the isolation and propagation of KSHV from KS-positive skin biopsy specimens. The cells are highly sensitive to KSHV replication, as cytopathic effects in culture are prominent 3 to 4 days following viral adsorption.
Growth Characteristics: Adherent cells. Split 1:5 twice weekly, or when they reach 70% confluency.
Growth Medium: DMEM medium supplemented with 10% fetal bovine serum
ARP-3553 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-3553 contains
approximately 5.7 × 106 cells in fetal bovine serum containing10% dimethyl sulfoxide (DMSO). Please refer to the
appropriate data sheet for lot-specific information.
K. E., et al. “Propagation of a Human Herpesvirus from AIDS-Associated Kaposi’s
Sarcoma.” N. Engl. J. Med. 336 (1997):
163-171. PubMed: 8988896.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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