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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-497 is culture of HSB-2 cells, a human T cell lymphoblastoid line derived from the peripheral blood buffy coat of a patient with acute lymphoblastic leukemia and propagated as tumors in newborn syrian hamsters.
Growth Characteristics: Cells grow in suspension with a lymphoblast-like morphology. Thaw the cells rapidly in a 37°C water bath, inverting the vial periodically. Transfer the thawed cells into a T-25 flask in 10 mL of growth medium. To dilute out the DMSO, stand the flask upright and incubate overnight. The next day, carefully aspirate off most of the medium and replace it with fresh culture medium. Once the cells are established in culture, maintain them at 1 × 106 to 2 × 106 cells per mL and split 1:2 to 1:3 with fresh medium every 2 days.
Growth Medium: RPMI 1640 medium supplemented with 10% fetal bovine serum
ARP-497 is negative for bacteria, Mycoplasma, yeast and mold.
Each vial of ARP-497 contains
approximately 1.3 × 107 cells in growth medium supplemented with 10% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the
attached file(s) for additional information.
Z., et al. “Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative
Disorders.” Science 234 (1986): 596-601. PubMed: 2876520.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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