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colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-9850 is a Jurkat-based cell line containing a full-length integrated human immunodeficiency virus 1 (HIV-1) genome that expresses GFP upon activation. The genome generates incomplete virions due to a frameshift in env.
ARP-9850 was derived from Jurkat cells infected with the packaged retroviral construct HIV-R7/E-/GFP, which is full length HIV-1 genome with a non-functional env due to a frameshift, and GFP in place of the nef gene. The full-length construct secretes incomplete viral particles (capsids). The cells express low to undetectable levels of GFP under basal conditions. ARP-9850 is suited to study HIV latency and reactivation.
Growth Characteristics: ARP-9850 grows in suspension with a small, spherical morphology, usually singly but some clumping has been noted. There are no special requirements for thawing and
reestablishing the culture. Split cultures at 1:3 at 1 × 106 cells per mL.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U per mL penicillin G and 100 µg per mL streptomycin
ARP-9850 is negative for bacteria, Mycoplasma and fungi.
Each vial of ARP-9850 contains
approximately 4.7 × 106 cells in fetal bovine serum containing 10% dimethyl sulfoxide
(DMSO). Please refer to the attached file(s) for additional information.
A., D. Bisgrove and E. Verdin. “HIV Reproducibly Establishes a Latent Infection
after Acute Infection of T Cells in Vitro.” EMBO J. 22 (2003):
1868-1877. PubMed: 12682019.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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