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Quantity limit per order
for this item is 1. This item can be ordered twice a year. Orders over this
limit will be sent to NIAID for approval before shipment.
ARP-151 is an HIV-1
LTR lacZ (pHIVlacZ) reporter vector that contains the complete U3
region and approximately 75 base pairs of the R region of the HIV-1 long
terminal repeat (LTR). The reported vector also includes a trans-activation
response element (TAR) which drives the Escherichia coli (E.
coli) lacZ gene.
reporter vector contains the 3.2 kb lacZ gene from pCH110,
which was removed by restriction digestion with HindIII and BamHI and ligated in
place of the CAT gene in the pUR3III-CAT vector (BEI Resources ARP-330).
ß-galactosidase assays show quite high levels of expression in human embryonic
teratocarcinoma cells or activated monocyte-macrophage lines.
contains no BamHI site. Digestion
of the vector with KpnI can produce an unusual
cleavage pattern, due to star activity, if the buffer conditions are not
correct, enzyme concentration is too high, or the digest is done on a miniprep.
When performing a digestion on a mini-prep, use Asp718 instead of KpnI.
vial of ARP-151 contains 1 mL of E. coli,
strain HB101 transformed with pHIVlacZ (approximately 1.6 x 109 ampicillin-resistant transformed bacteria). Please refer
to the appropriate data sheet for lot-specific information.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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