Disclaimer: You are now leaving www.HIVReagentProgram.org and are going to a website that is not operated by NIH HIV Reagent Program. We are not responsible for the content or availability of linked sites.
ABOUT THIRD PARTY LINKS ON OUR SITE: NIH HIV Reagent Program offers links to other third party websites that may be of interest to our website visitors. The links provided in our website are provided solely for your convenience and may assist you in locating other useful information on the Internet. When you click on these links, you will leave the NIH HIV Reagent Program website and will be redirected to another site. These sites are not under the control of NIH HIV Reagent Program. NIH HIV Reagent Program is not responsible for the content of linked third party websites. We are neither an agent for these third parties nor do we endorse or guarantee their products. We make no representation or warranty regarding the accuracy of the information contained in the linked sites. We suggest that you always verify the information obtained from linked websites before acting upon this information. Please read third party privacy and security policies closely as these may be different than NIH HIV Reagent Program policies. If you have any questions or concerns about the products and services offered on linked third party websites, please contact the third party directly.
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-151 is an HIV-1 LTR lacZ (pHIVlacZ) reporter vector that contains the complete U3 region and approximately 75 base pairs of the R region of the HIV-1 long terminal repeat (LTR). The reported vector also includes a trans-activation response element (TAR) which drives the Escherichia coli (E. coli) lacZ gene.
The reporter vector contains the 3.2 kb lacZ gene from pCH110, which was removed by restriction digestion with HindIII and BamHI and ligated in place of the CAT gene in the pUR3III-CAT vector (BEI Resources ARP-330).
Standard ß-galactosidase assays show quite high levels of expression in human embryonic teratocarcinoma cells or activated monocyte-macrophage lines.
pHIVlacZ contains no BamHI site. Digestion of the vector with KpnI can produce an unusual cleavage pattern, due to star activity, if the buffer conditions are not correct, enzyme concentration is too high, or the digest is done on a miniprep. When performing a digestion on a mini-prep, use Asp718 instead of KpnI.
Each vial of ARP-151 contains 1 mL of E. coli, strain HB101 transformed with pHIVlacZ (approximately 1.6 x 109 ampicillin-resistant transformed bacteria). Please refer to the appropriate data sheet for lot-specific information.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers. While we use reasonable efforts to include accurate and up-to-date information on this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.