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-70°C or colder
Quantity limit per order
for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-2134 [CAT Reporter Vector (pIL-2r)] is a reporter
vector containing a synthetic 26 base
pair oligonucleotide containing the alpha subunit of the IL-2 receptor (IL-2Rα)
NF-κB binding site (-268 through -243) was cloned into the SalI site of plasmid Δ56, a minimal c-fos promoter/CAT reporter plasmid. The synthetic κB
oligonucleotide sequence is: (-268) GGGGAATCTCCCTCTCCTTTTATGGG (-243). The κB
enhancer drives increased CAT expression in transfected S194 myeloma and 3T3
cells. This activity is further increased by LPS stimulation of these B-cell
lines. CAT expression in Jurkat T cells occurs after PMA stimulation, but does
not occur in the absence of mitogen induction. Double digestion of the plasmid
with EcoRI and HindIII produces a 500 kb fragment containing the insert as well
as 2650 kb and 2130 kb fragments. Digestion with HincII produces a 1750
kb fragment containing the insert, as well as 2900 kb, 370 kb and 240 kb
vial of ARP-2134 contains 1 mL of E. coli, strain DH5α transformed with pIL-2r. Please refer to the attached file(s) for more information.
Pierce, J. W., M. Lenardo and
D. Baltimore. “Oligonucleotide that Binds Nuclear Factor NF-κB Acts as a
Lymphoid-Specific and Inducible Enhancer Element.” Proc. Natl. Acad. Sci. USA 85 (1988):1482-1486. PubMed:
Gilman, M. Z., R. N. Wilson and
R. A. Weinberg. “Multiple Protein-Binding Sites in the 5'-Flanking Region
Regulate C-fos Expression.” Mol.
Cell. Biol. 6 (1986): 4305-4316.
for-profit institutions or who intend commercial use of this reagent must
contact the NIH Office of Technology Transfer, Email: NIAIDAIDSReagent@niaid.nih.gov,
before the reagent can be released. Please specify the name and a description
of the intended use of the reagent.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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