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protected from moisture
Ψ -Moloney Murine Leukemia
virus (MLV) DNA (from Richard Mann) was cloned into the SV40 expression vector
pSV7d at the EcoRI site. Restriction
sites in the MLV sequence can be found in the Appendix to Volume 2 of the Cold
Spring Harbor Tumor Virus Book. The mouse flanking sequences present on either
side of the provirus have not been sequenced.
This construct is 13,966 base pairs including the insert;
the insert is 11,606 base pairs. The cloning vector used to create pSV-Ψ-MLV-env-was pSV7d and the
resulting vector is ampicillin resistant. For additional details please refer
to the provided vector map and sequence files.
This plasmid is used to produce MLV retroviral vectors
using the method of Landau and Littman. In the original method, COS cells were
transfected with this plasmid and an amphotropic MLV env vector. Higher virus titers can be obtained by transfecting 293
cells instead of COS cells, and with the VSV-G expression vector instead of
Plasmids can be propagated in STBL2 cells and grown at
37°C. Larger plasmids may benefit from growth at 30°C. This construct may also
be grown in other competent cells.
Each vial of ARP-3422 contains
approximately 5 µg of dried purified DNA stabilized in DNAstable®PLUS. Please refer to the appropriate
data sheet for lot-specific information.
N. R. and D. R. Littman. “Packaging System For Rapid Production Of Murine
Leukemia Virus Vectors With Variable Tropism.” J. Virol. 66 (1992): 5110-5113. PubMed:1321291.
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Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
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as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
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For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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