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protected from moisture
is a HIV-1-based reporter vector that is both replication-competent and
pathogenic in vivo. Virus
produced from pNL-r-HSAS infects cells expressing both CD4 and CXCR4. Infected
cells express surface heat stable antigen (HAS) which is detectable by flow
pNL-r-HSAS contains a 267 base pair fragment from the ORF
of HSA that was inserted into an introduced XbaI
site (nt 5625) and an existing EcoRI
site (nt 5743) of cloning vector pNL4-3. This site is within the NL4-3 vpr gene, which was paritally deleted
during the digests. In addition, the start of vpr and two potential start sites in the 3' end of vif are silenced by site-specific
mutagenesis. The insert was cloned in the 5'-3' orientation. The murine HSA
insert was derived from the plasmid pSL87c4-1. The insert contains the HSA ORF
and 36 extraneous bases from the original plasmid. HSA expression is driven by
the HIV-1 long terminal repeat (LTR).For additional details please refer to the
provided plasmid map and sequence file.
Each vial of ARP-4101 contains
approximately 2.45 µg of dried purified DNA stabilized in DNAstable®PLUS. Please refer to the appropriate
data sheet for lot-specific information.
Jamieson, B. D. and J. A. Zack. “In Vivo Pathogenesis of a Human Immunodeficiency Virus Type 1
Reporter Virus.” J Virol. 72 (1998): 6520-6526.
Adachi, A., et al. “Production
of Acquired Immunodeficiency Syndrome-Associated Retrovirus in Human and
Nonhuman Cells Transfected with an Infectious Molecular Clone.” J Virol.59 (1986): 284-291. PubMed:3016298.
Kay, R., F. Takei and R. K. Humphries. “Expression Cloning
of a cDNA Encoding MI/J11d Heat-Stable Antigens.” J Immunol. 14 (1990): 1952-1959. PubMed: 2118158.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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