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-70°C or colder
contains the 3' long terminal repeat
(LTR) region from an HIV-1 subtype F patient isolate upstream of the luciferase
gene. The LTR was shown to be functional using the subtype B HIV-1, LAI, Tat protein.
The LTR region (nt -147 to +63 relative to the transcription start site) from a
subtype E patient isolate was PCR-amplified and exchanged into pBlue3'LTR-luc-B
(BEI Resources ARP-4788) in place of the HIV-1, LAI, LTR. It contains two NFκB
enhancers, three Sp1 binding sites, the TATA box and the TAR TNA hairpin motif.
The construct is 5720 base pairs and is also referred to as pBlue3'LTR-luc-F.
ARP-4788-K02013.1 (subtype B)
ARP-4789-AF127567 (subtype C)
ARP-4790-AF127569 (subtype D)
ARP-4791-AF127570 (subtype E)
ARP-4792-AF127571 (subtype F)
ARP-4793-AF127572 (subtype G)
vial of ARP-4792 contains ampicillin resistant Escherichia coli (E. coli) DH5α transformed
with pBlue3'LTR-luc-F in LB broth with 20% glycerol. Please refer
to the appropriate data sheet for lot-specific information.
Jeeninga, R. E., et al.
“Functional Differences Between the Long Terminal Repeat Transcriptional
Promoters of Human Immunodeficiency Virus Type 1 Subtypes A Through G.” J. Virol. 74 (2000):3740-3751.
Klave, B. and B. Berkhout. “Comparison
of the 5' and 3' LTR Promoter Function in the Human Immunodeficiency Virus.” J. Virol. 68 (1994):3830-3840.
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