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HRP-20053 is a full-length molecular clone of
infectious and replication-competent simian-human immunodeficiency provirus. This clone contains an amino acid residue at
Env position 375 that supports virus entry and replication in primary rhesus
CD4 T cells. SHIV.1086.375W.dCT
is an isogenic mutant of SHIV.1086.375S.dCT generated by changing 1086 Env375 residue (GenBank: MW410739) from Ser to Trp. SHIV.1086.375W.dCT
showed increased infectivity and replication kinetics in vitro in Indian
rhesus macaque CD4+ T cells and in vivo in Indian rhesus
macaques. The plasmid
encodes full-length, replication-competent SHIV in a pBR322 backbone. The beta-lactamase gene,
bla, provides transformant selection
through ampicillin resistance in Escherichia
coli (E. coli). The resulting
size of the plasmid is 14,890 base pairs. The
plasmid sequence and map are provided on the NIH HIV Reagent Program webpage.
Note: Bacterial transformation and large-scale preparation of plasmid DNA are tricky due to plasmid instability. The contributors have developed a protocol to prevent this by growing bacteria for shorter time periods and at lower temperatures.Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to transform the plasmid in E. coli prior to mammalian expression.
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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