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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be
ordered twice a year. Orders over this limit will be sent to NIAID for approval
ARP-103 is a human T lymphoblastoid cell
line (originally referred to as
CEM-T4) isolated from primary human embryonic kidney tissue and transformed by
sheared human adenovirus type 5 DNA. CEM CD4+ is a naturally isolated
subclone of the CEM line with high levels of surface CD4 expression.
Growth Characteristics: Cells are grown
in suspension with a lymphoblast-like morphology. An inoculum of 105 cells per mL will
increase four to five fold in 4 to 5 days when incubated at 37°C, providing pH
is maintained at 7.0 and fresh medium is added every other day. Maintenance of
the cell population at 106 cells per mL is optimal for growth.
Propagation Medium: Minimal Essential Medium (MEM)
supplemented with 10% fetal bovine serum and without antibiotics
ARP-103 is negative for mycoplasma, bacteria and fungi.
Each vial of ARP-103 contains approximately 3.8 x 106 cells in Minimal
Essential Medium (MEM) supplemented with 10% fetal bovine serum and 10% dimethyl
sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific
Foley, G. E., et al. “Continuous Culture of Human
Lymphoblasts from Peripheral Blood of a Child with Acute Leukemia.”Cancer18 (1965): 522-529. PubMed:
When applying for permits and forms please:
Do NOT reference NIH HIV Reagent Program or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your NIH HIV Reagent Program registration.
Information about permits is provided as a courtesy to NIH HIV Reagent Program customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by NIH HIV Reagent Program, please select this link: Compliance Requirements.
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