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Welcome to NIH HIV Reagent Program

Welcome to NIH HIV Reagent Program

The NIH AIDS Reagent Program has been transitioned to the NIH HIV Reagent Program which is managed by American Type Culture Collection (ATCC). We want to ensure your continued use of the materials within the NIH HIV Reagent Program with minimal impact to you.   The NIH HIV Reagent Program materials are provided at no cost to registered researchers (shipping charges apply).

Welcome to NIH HIV Reagent Program
HIV virus cells. Scientifically accurate human immunodeficiency virus (HIV) close-up view. Acquired immunodeficiency syndrome AIDS 3D medical illustration. HIV viral particles with membrane, proteins. Corona Borealis Studio/Shutterstock

HIV-1 Latent J-Lat Series Cells

J-Lat cells were generated by infecting Jurkat cells with an HIV-1 pseudotyped virus, where Nef was replaced with GFP, and clones were characterized (ARP-9846 to ARP-9850). Similar latent cells were also established with a simpler retroviral vector, Tat-IRES-EGFP, under the control of LTR, and clones were characterized (ARP-9851 to ARP-9856).  All clones can be activated by most Latency Reversing Agents. For analysis, GFP can be measured by flow cytometry or p24 ELISA of culture supernatants.

 

HIV-1 Latent J-Lat Series Cells
HIV-1 Dual Reporter pRGH-WT. Image credit: (HIV virus. Liya Graphics/ Shutterstock)

Coming Soon: HIV-1 Dual Reporter pRGH-WT

Red-Green-HIV-1 (RGH) molecular clone (ARP-12427), is HIV-1 B-LAI Δenv double-labeled with eGFP, as an in-frame Gag fusion, and CMV-driven mCherry, located in place of Nef. Therefore, it allows the detection of infected cells independently of long terminal repeat (LTR) activity and serves as a sensitive tool to help in analyzing HIV-1 latency on a single-cell level. Related materials include ARP-12428 (pRGH-Integrase defective), ARP-12429 (pRGH-ΔCMV) and ARP-12430 (pRGH-ΔU3(3’).

Coming Soon: HIV-1 Dual Reporter pRGH-WT
HIV-1 Env Expression Vectors Panel.  Image credit: (A domain (gp120, violet) of the HIV-1 envelope protein interacts with the CD4 receptor (dark blue) and a chemokine receptor (CCR5, light blue) of an immune cell. Source: PDB entry 6MEO. 3d rendering. Juan Gaertner/Shutterstock)

HIV-1 Env Expression Vectors Panel

This panel of full-length functional gp160 expression vector clones from 56 international HIV-1 primary isolates representing six clades (A, B, C, D, AE, and AG) is a great tool for vaccine and functional immune response studies, which can be used to generate pseudoviruses and naturalization assays. These clones are available in the NIH HIV Reagent Program catalog individually (ARP-13489 to ARP-13523) or as part of a panel set (ARP-13467).

Recombinant HIV-1Tat Protein
Peptide libraries, HIV-1 proteins.  Image credit:  3D Illustration of Immune System cells attacking a HIV Virus. Spectral-Design/Shutterstock.

Peptide libraries, HIV-1 proteins

Peptide pool, HIV-1 subtype B Env (ARP-12540), Gag (ARP-12425), Nef (ARP-12545), Pol (ARP-12438), Vpr (ARP-12860) and Rev (ARP-6445) are of the most requested reagents in the HRP catalog. These peptides are produced as 15-mers with 11 aa overlaps so that T-cell stimulation is optimized while the chance of missing T-cell epitopes is minimized. Peptide arrays for the same HIV-1 viral proteins are also available (ARP-9480, ARP-8117, ARP-5189, ARP-6208, ARP-6447).

Peptide libraries, HIV-1 proteins
How to Register

How to Register

For information and forms regarding registration with the NIH HIV Reagent Program, click here.

How to Register
How to Order

How to Order

After your registration application has been approved by NIAID and ATCC, you will receive a login name and password. After logging in, find the reagent you need using the search function. Select the items from the search results to see the product detail pages. After you read the product detail page description, click the "Add to cart" button to place the item into your shopping cart. The NIH HIV Reagent Program materials are provided at no cost to registered researchers (shipping charges apply). For additional information, click here.

How to Order
Contact Us

Contact Us

Our staff are available to assist you, please contact us at contact@HIVReagentProgram.org.

Contact Us


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If you have Comments, Questions and Suggestions regarding the NIH HIV Reagent Program please contact us.

Register

The NIH HIV Reagent Program acquires, authenticates, produces and distributes a variety of materials within several biosafety levels and classifications. We understand that some researchers may only choose to order non-infectious research grade reagents through the NIH HIV Reagent Program.

Knowledge Base

The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the NIH HIV Reagent Program and reagents through questions and answers.